Journal: Advanced Biomedical Research

Article Title: Effect of miR-4270 on Tumorigenicity in Gastric Cancer Stem Cells via the Wnt Signaling Pathway

doi: 10.4103/abr.abr_309_24

Figure Lengend Snippet: The effect of hsa-miR-4270 on angiogenesis and MMP activity. HUVECs were cultured on Matrigel for 24 h as (a) untreated cells, (b) hsa-miR-4270 inhibitor, and (c) hsa-miR-4270 mimic. Images show that the hsa-miR-4270 inhibitor repressed angiogenesis; however, tube-like structures were formed on Matrigel treated with hsa-miR-4270 mimic and untreated cells. (d) GCSCs treated with microRNA-4270 mimic as well as untreated GCSCs had no negative effect on MMP activities for 48 h. (e) Counting of migrated cells from the microporous membrane in the Transwell plate after 48 h indicated 531, 1839, and 1114 cells migrated in GCSCs treated with the hsa-miR-4270 inhibitor, mimic, and untreated GCSCs, respectively (values are mean ± SEM, P < 0.05 vs. control group, ANOVA analysis)

Article Snippet: The effect of hsa-miR-4270 inhibitor and mimic on angiogenesis was assessed using human umbilical vein endothelial cells (HUVECs) (National Cell Bank of Iran affiliated to Pasteur Institute of Iran) and ECM gel (Sigma-Aldrich).

Techniques: Activity Assay, Cell Culture, Membrane, Control

Journal: Asian Pacific Journal of Cancer Prevention

Article Title: Quercetin Effects on Cell Cycle Arrest and Apoptosis and Doxorubicin Activity in T47D Cancer Stem Cells

doi: 10.31557/apjcp.2022.23.12.4145

Figure Lengend Snippet: Figure 1. Isolation of CD44+/CD24- CSCs from T47D Cancer Cells by MACs. The CD44+/CD24- CSCs of T47D Cancer Cells were Isolated by CD24 and CD44 Magnetic beads Using the Miltenyi's MACS System. The parental T47D cancer cells (A) and its isolated CD44+/CD24- CSCs (B) were subjected to immunostaining with anti-CD44- FITC and anti-CD24-PE antibodies and analyzed by flow cytometry to determine the percentage of CD44+/CD24- cells within each population of T47D cells.

Article Snippet: The human breast cancer cell line T47D (purchased from National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran), were grown in RPMI-1640 medium supplemented with 10% heat-inactivated (50°C, 30 minutes) fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 5% CO2 and 95% air in a humidified 37°C incubator.

Techniques: Isolation, Magnetic Beads, Immunostaining, Flow Cytometry

Journal: Asian Pacific Journal of Cancer Prevention

Article Title: Quercetin Effects on Cell Cycle Arrest and Apoptosis and Doxorubicin Activity in T47D Cancer Stem Cells

doi: 10.31557/apjcp.2022.23.12.4145

Figure Lengend Snippet: Figure 3. Effects of Quercetin on Doxorubicin Cytotoxicity in T47D Cells. The T47D cells were co-treated with different concentrations of Quercetin and Doxorubicin to determine cell proliferation using MTT assay. The results were expressed as mean±SE of three independent experiments in quadruplicates layout for each concentration. *denotes p<0.001 for significant difference between treatments in comparison to control RPMI.

Article Snippet: The human breast cancer cell line T47D (purchased from National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran), were grown in RPMI-1640 medium supplemented with 10% heat-inactivated (50°C, 30 minutes) fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 5% CO2 and 95% air in a humidified 37°C incubator.

Techniques: MTT Assay, Concentration Assay, Comparison, Control

Journal: Asian Pacific Journal of Cancer Prevention

Article Title: Quercetin Effects on Cell Cycle Arrest and Apoptosis and Doxorubicin Activity in T47D Cancer Stem Cells

doi: 10.31557/apjcp.2022.23.12.4145

Figure Lengend Snippet: Figure 5. Cell Cycle Alteration in T47D Cell Line and Isolated CD44+/CD24- CSCs. The T47D cells (A) and its isolated CD44+/CD24- CSCs (B) were treated with Doxorubicin and Quercetin alone and in combination for 48h to determine cell cycle distribution pattern using DAPI staining by flow cytometry analysis. Data are presented as the mean±SE of three independent experiments. * denotes p<0.05 for significant difference between treatments in comparison to control RPMI.

Article Snippet: The human breast cancer cell line T47D (purchased from National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran), were grown in RPMI-1640 medium supplemented with 10% heat-inactivated (50°C, 30 minutes) fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 5% CO2 and 95% air in a humidified 37°C incubator.

Techniques: Isolation, Staining, Flow Cytometry, Comparison, Control

Journal: Asian Pacific Journal of Cancer Prevention

Article Title: Quercetin Effects on Cell Cycle Arrest and Apoptosis and Doxorubicin Activity in T47D Cancer Stem Cells

doi: 10.31557/apjcp.2022.23.12.4145

Figure Lengend Snippet: Figure 6. Apoptosis Induction in T47D Cell Line and Isolated CD44+/CD24- CSCs. The T47D cells (A) and its isolated CD44+/CD24- CSCs (B) were treated with Doxorubicin and Quercetin alone and in combination for 48h to evaluate induction of apoptosis using Annexin V-FITC/PI double-staining by flow cytometry. The percentage of early and late apoptotic cells were presented for each treatment group. Data are presented as means ± SE of three independent experiments. *denotes p<0.001 for significant difference between treatments in comparison to control RPMI.

Article Snippet: The human breast cancer cell line T47D (purchased from National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran), were grown in RPMI-1640 medium supplemented with 10% heat-inactivated (50°C, 30 minutes) fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 5% CO2 and 95% air in a humidified 37°C incubator.

Techniques: Isolation, Double Staining, Flow Cytometry, Comparison, Control

Journal: The Plant Pathology Journal

Article Title: Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR

doi: 10.5423/PPJ.OA.04.2019.0086

Figure Lengend Snippet: Population of Heterodera species used in this study

Article Snippet: H. mani , - , NCBI , MG523095 ( CO I) , Primer design.

Techniques:

Journal: Mammalian Genome

Article Title: Mouse genome annotation by the RefSeq project

doi: 10.1007/s00335-015-9585-8

Figure Lengend Snippet: Overview of NCBI’s eukaryotic annotation pipeline from http://www.ncbi.nlm.nih.gov/genome/annotation_euk/process/#process . Briefly, genomic sequences are repeat masked ( gray ), and transcripts ( blue ), proteins ( green ), RNA-seq reads ( orange ), and curated RefSeq sequences ( pink ) are aligned to the genome. Based on these alignments, gene model predictions are calculated ( brown ), best models are selected, named and accessioned ( purple ), and finally annotation products are released publicly ( yellow ). During re-annotations, models and genes are given special attention and are tracked from one annotation release to the next

Article Snippet: The annotation report for NCBI’s most recent Mus musculus Annotation Release 105 (a February 2015 update for the GRCm38.p3 and Celera assemblies) is available at http://www.ncbi.nlm.nih.gov/genome/annotation_euk/Mus_musculus/105/ .

Techniques: Genomic Sequencing, RNA Sequencing

Journal: Mammalian Genome

Article Title: Mouse genome annotation by the RefSeq project

doi: 10.1007/s00335-015-9585-8

Figure Lengend Snippet: Graphical display of mouse RefSeq transcripts using NCBI’s Gene Resource. a Genomic context. Coordinates on multiple mouse genome assemblies and a graphical display of the location and orientation of genes neighboring the Fst (GeneID: 14313) locus are shown here. b Genomic regions, transcripts, and products. Tracks displayed with the default settings are indicated with red arrows . The configure button ( red circle ) may be used to customize tracks. c Zoom and pan features allow easy identification of differences between transcript variants. Quantitative RNA-seq intron features data are displayed in this view. d Shown here is a subset of the links and related information displayed in the sidebar of each Gene record

Article Snippet: The annotation report for NCBI’s most recent Mus musculus Annotation Release 105 (a February 2015 update for the GRCm38.p3 and Celera assemblies) is available at http://www.ncbi.nlm.nih.gov/genome/annotation_euk/Mus_musculus/105/ .

Techniques: RNA Sequencing

Journal: The Plant Pathology Journal

Article Title: Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR

doi: 10.5423/PPJ.OA.04.2019.0086

Figure Lengend Snippet: Population of Heterodera species used in this study

Article Snippet: H. latipons , - , NCBI , MG523117 ( CO I) , Primer design.

Techniques: